Saturday, September 22, 2018

Testing 3D Printed Orbital Shaker V 2.0

September 23, 2018 | ATL 

The first application we've put the 3D printed orbital shaker to work on was agitating containers with samples in it for this red cabbage pH protocol. The protocol is simple, takes just minutes to do, and depending on the sample you are testing, can work instantly or require a bit of agitation which you could easily do by hand, but we have an orbital shaker so why not use it? 

One sample (pictured below, left) was actually homemade soap. The middle is coffee, and the right is regular tap water. We also later tested lime juice which instantly turned the cabbage juice red-pink.  


There are scales online showing the different colors red cabbage juice will change depending on what pH it is exposed to. The image below was taken from here.



We've been making DIYbio lab equipment mainly just to make it and put it out there for others to use, modify, and improve on. The designs for our equipment are freely available on Thingiverse here. We haven't been using it for too many applications of our own yet, but we have used the centrifuge to extract DNA from rice, and now this pH protocol shows some promise. 

What would you use your shaker for? 

Follow ProgressTH.org on Facebook here, Instagram here, or on Twitter here. We also put all of our 3D printed models online for free at Thingiverse.com here.

Saturday, July 14, 2018

Found! 3D Printed Rocker-style DIYbio Shaker on Thingiverse

July 14, 2018 | ATL

This 3D printed rocker-style laboratory shaker was designed by Akshay Dhamankar, an engineer/maker from Buffalo, New York, USA who shared the design on Thingiverse.



We like the use of ball bearings, stepper motor driven by a stepper driver and Arduino and the simple potentiometer setup. This is very similar to our orbital shaker and means building this particular design or one similar to it can be done with what we have on hand.



We ultimately want to make something similar (but smaller) to the GE Wave Bioreactor System which is also a rocker-style laboratory shaker with heating and other options built in. So Akshay's rocker-style system gives us a good reference point for informing our design meaning we won't have to start from scratch.

This is the beauty of the online opensource 3D printing community. Everyone makes designs that inspire others and it all moves things forward. Together, we're all benefiting from lessons we learn with each design and implementation.

Follow ProgressTH.org on Facebook hereInstagram here, or on Twitter here. We also put all of our 3D printed models online for free at Thingiverse.com here.

Monday, July 9, 2018

3D Printed Orbital Shaker: Exploded View

July 10, 2018 | ATL

Here is an exploded diagram of the DIYbio 3D printed orbital shaker.

The Thingiverse files include potentiometer holders for two kinds of potentiometers common at electronics stores (here and here). There are also feet you can add to the bottom. Hot glue doesn't seem robust enough, so friction welding or super glue might be better options.

This exploded view also includes the newer M5 bolt bar system.

The diagram is a stop gap until we do some videos or tutorials on assembly. 

Follow ProgressTH.org on Facebook hereInstagram here, or on Twitter here. We also put all of our 3D printed models online for free at Thingiverse.com here.

Monday, July 2, 2018

3D Printed Lab Blocks

July 2, 2018 | ATL

This is a very simple block for holding various sizes of tubes commonly used in the lab. The file is available for free on Thingiverse to 3D print. Just flip them around to access different sized holes for different sized tubes. We've numbered the smaller holes to make it easier to keep track of what's what.


We've been using them recently to test on our orbital shaker v2.0. The rack system was doing a good job holding them in place as the tubes were shaken. We've got some improvements to make on the rack system and we will be updating that soon. 

Follow ProgressTH.org on Facebook hereInstagram here, or on Twitter here. We also put all of our 3D printed models online for free at Thingiverse.com here

Sunday, July 1, 2018

3D Printed Orbital Shaker v2.0 on Thingiverse

July 1, 2018 | ATL 

Our 3D printed DIYbio laboratory orbital shaker v2.0 is finally completed and working. We've put all the files up on Thingiverse. Over time we will improve the documentation on it, including videos on how to assemble it. But it is essentially just a larger version of our original, smaller prototype here.


Below is a 5 second video of the orbital shaker in action. We've also tested it with up to 1kg of weight in the form of a 900mL (30oz) jar filled with water.


The rack system can probably be redesigned to be a little higher. We tested the shaker with our 3D printed lab blocks for shaking multiple test tubes at once at maximum speed with satisfactory results.

Follow ProgressTH.org on Facebook hereInstagram here, or on Twitter here. We also put all of our 3D printed models online for free at Thingiverse.com here

Tuesday, June 26, 2018

3D Printing on DIYbio Orbital Shaker Completed

June 27, 2018 | ATL 

Here is the DIYbio 3D printed orbital shaker v2.0 fitted together after 3D printing was finished up. The motion control works perfectly. We still have to wire it up and friction weld (or glue) the ball bearings in place.


The platform is 250mm wide and 150mm deep. It includes an adjustable bar system for holding containers in place. We might have to work on the knobs a little more, but they do work as is.

V2.0 is pictured next to our original prototype (right). More information and a video of the original prototype in action can be found here. It was a bit top heavy and its platform lacked space for many real-world applications we wanted to try. V2.0 solves both problems.

Our DIYbio lab equipment projects are meant to spark interest in opensource biotech and start getting these tools into the hands of more innovators. For a look at all our previous projects now up on Thingiverse, see here

Follow ProgressTH.org on Facebook hereInstagram here, or on Twitter here. We also put all of our 3D printed models online for free at Thingiverse.com here

Sunday, June 24, 2018

How Big is Our Orbital Shaker V2.0?

June 24, 2018 | ATL 

Our version 2.0 of our 3D printed DIYbio orbital shaker is going to be much, much bigger than our first prototype. For our first prototype, check out all the files, images, and videos here on Thingiverse.



This is to make sure it can actually handle real-world applications like agitating etching solution for electronics or moving something around bigger than a single mason jar.



To get an idea of just how big it is, check out the motion control cradle in relation to our first prototype. We've also included a graphic from SketchUp with dimensions of what 2.0 will look like when it's finished and how big it will be.

Follow ProgressTH.org on Facebook hereInstagram here, or on Twitter here. We also put all of our 3D printed models online for free at Thingiverse.com here